![]() ![]() The observation that haemoglobin could crystallize on dilution of unpurified red cell lysate 21 facilitated the advent of protein X-ray crystallography 22. In-vivo protein crystallization is rare with mammalian examples including insulin 18 and Charcot–Leyden crystals 19, 20. As PAK4 does not appear to use adaptors, we investigated the possibility that Inka1, first identified as a PAK4-binding protein in frogs 17, might fulfill this role. The binding of Cdc42 can serve to activate PAK4 in cells, but it is unclear whether there is any autophosphorylation event associated with this activation 13. ![]() 13) and kept in check through the intramolecular association of the AID. Although PAK1 activation in vivo occurs through activation loop Thr-423 phosphorylation 16, it is notable that PAK4 is constitutively phosphorylated on Ser-474 (ref. This is consistent with the notion that PAK4 lacking residues 10–30 in the Cdc42/Rac interactive binding domain is active 15. ![]() We have shown that Cdc42 directly regulates PAK4 activity in mammalian cells through an auto-inhibitory domain (AID) that binds in a manner similar to pseudosubstrates 13, 14. The structural basis for PAK4’s preference for serine containing substrate sites has recently been elucidated 12. PAK4 is a kinase with strong links to cellular transformation and cancer metastasis, as reviewed 11. The loss of PAK4 prevents proper polarization and thus formation of the endothelial lumen 9, consistent with defects seen in PAK4−/− mice 10. The phenotype of PAK4-null mice, which is embryonic lethal, involves defects in the fetal heart as well as in neuronal development and axonal outgrowth 8. This is consistent with PAK4 functioning primarily at cell–cell contacts in mammalian cells 6, with Cdc42 also being required for adherent junction formation 7. PAK4-like kinases are ubiquitously expressed in metazoans but are not found in protozoa or fungi 4, 5. At the same time, fans get new ways to engage with their favorite artists directly and even own a music or video of their creations.Mammalian PAK isoforms are categorized into two groups on the basis of their structural and biochemical features: the conventional or group I PAKs in human comprise PAKs1–3, whereas the group II PAKs (PAK4–6) are encoded by three genes in mammals 1, 2, 3. Generate instant revenue by selling master or publishing copyrights of their music or movie as NFTs.īesides licensing rights, film makers and musicians can junctions special utility into their NFTs, such as privileged access to tickets, backstage experiences, and much more.Įither way, artists can generate new income streams and free themselves from the clutches of streaming providers. Enabling film makers, musicians directly monetize their movie and music through yield-bearing NFTs, which generate yield through royalties - actual real-world cash flow generated by the licensed use of an artist’s music and/or movie. While producers and other intermediaries would still have their role, the overall monetization process could become a whole lot more just and transparent. NFT MMM have the powerful ability to establish a direct connection between artists and fans. They are bought and sold online, frequently with cryptocurrency, and they are generally encoded with the same underlying software as many cryptos. An NFT is a digital asset that represents real-world objects like movie, music, and videos. NFTs are shaking the entertainment world, democratizing NFT (Non-Fungible Token) and bringing them to real theatre aficionados is the need of the hour. This is a unique and creative solution offering to its community, which see a great benefit for both artists, fans & corporates.ĪBQQ jointed with STAREASTnet, who envisions to be the multi-chain decentralized NFT marketplace for unique physical, digital download with a goal to bring NFT’s to mainstream movie and music aficionados. STAREASTnet is soon launching its first NFT movie and music marketplace with the option to buy physical, digital download or both, in one place with NFTs. (OTCQB: ABQQ), an intellectual property (IP) and movie investment and licensing firm, announces partnership of STAREASTnet, a leading Chinese entertainment artists network ABQQ will hold 40% stake of STAREASTnet NFT Movies and Music Marketplace. ![]()
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